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Comparison of Structure and Transport Function Between Calu-3 and Other Lung Epithelial Cells

In this study, researchers conducted side-by-side experiments to compare the structure and transport function of three lung epithelial cell lines, which include: Calu-3, normal human bronchial primary cells (NHBE), and NL-20. The three cell lines were cultured in air-liquid interface (ALI) culture conditions for 20 days. Results from various assays found that Calu-3 and NHBE cells exhibited lower paracellular permeability and higher TEER values compared to NL-20 cells. Calu-3 cells were found to form intact cell monolayers, whereas NHBE and NL-20 cells formed multilayers. Calu-3 also exhibited cytometric features for mimicking an in vivo airway epithelium. These findings suggest that the monolayers formed in Calu-3 cells have the potential to be used as functional cell barriers for the transport of lung-targeted drugs. [LINK]


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