Lung cancer is one of the more prominent cancers known to the medical field, with non-small cell lung carcionma (NSCLC) being the most common. Calu-3 cells come from such a cancer, and are useful tools in medical research evaluating the effects of genetic modification on cell proliferation. With the proper reagents, transfected siRNA, DNA, and RNA sequences can affect cell functionality and lead to important results that inform preclinical testing and in vitro effects of gene therapies and drugs.
Calu-3 cells are characterized as a human bronchial cell line composed of serous cells of airway submucosal glands that are responsible for secreting electrolytes that facilitate airway hydration. This study analyzes the effect of epinephrine on Calu-3 electrolyte transport. Results found that epinephrine stimulated anion secretion through two different channels: cystic fibrosis transmembrane conductance regulator and a Ca2+ activated Chlorine channel, in turn altering mucociliary clearance. Treating Calu-3 cells with epinephrine also resulted in increased cAMP and Ca2+. [ LINK ]
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