In this study, researchers conducted side-by-side experiments to compare the structure and transport function of three lung epithelial cell lines, which include: Calu-3, normal human bronchial primary cells (NHBE), and NL-20. The three cell lines were cultured in air-liquid interface (ALI) culture conditions for 20 days. Results from various assays found that Calu-3 and NHBE cells exhibited lower paracellular permeability and higher TEER values compared to NL-20 cells. Calu-3 cells were found to form intact cell monolayers, whereas NHBE and NL-20 cells formed multilayers. Calu-3 also exhibited cytometric features for mimicking an in vivo airway epithelium. These findings suggest that the monolayers formed in Calu-3 cells have the potential to be used as functional cell barriers for the transport of lung-targeted drugs. [LINK]
The Calu-3 cell line was derived from the carcinomatous lung tissue of a 25 year old Caucasian male suffering from adenocarcinoma. Before the cell line was established the patient had received therapy with bleomycin , cytoxan, and adriamycin. The tumorigenic, epithelial cells of the Calu-3 cell line are now used to produce a transfection host model for various types of lung cancer as well as other biomedical research. The Calu-3 transfection reagents established by Altogen Biosystems allow an intracellular delivery of pDNA, mRNA and siRNA and the recombinant protein expression desired in certain research. Calu-3 Transfection Reagent (Lung Carcinoma Cells, HTB-55)
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