Calu-3 cells are characterized as a human bronchial cell line composed of serous cells of airway submucosal glands that are responsible for secreting electrolytes that facilitate airway hydration. This study analyzes the effect of epinephrine on Calu-3 electrolyte transport. Results found that epinephrine stimulated anion secretion through two different channels: cystic fibrosis transmembrane conductance regulator and a Ca2+ activated Chlorine channel, in turn altering mucociliary clearance. Treating Calu-3 cells with epinephrine also resulted in increased cAMP and Ca2+. [LINK]
The Calu-3 cell line was derived from the carcinomatous lung tissue of a 25 year old Caucasian male suffering from adenocarcinoma. Before the cell line was established the patient had received therapy with bleomycin , cytoxan, and adriamycin. The tumorigenic, epithelial cells of the Calu-3 cell line are now used to produce a transfection host model for various types of lung cancer as well as other biomedical research. The Calu-3 transfection reagents established by Altogen Biosystems allow an intracellular delivery of pDNA, mRNA and siRNA and the recombinant protein expression desired in certain research. Calu-3 Transfection Reagent (Lung Carcinoma Cells, HTB-55)
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