Calu-3 is a tumorigenic cell line that was established from the lung tissue a 25-year-old Caucasian male with adenocarcinoma. The patient had been treated with Cytoxan, Bleomycin, and Adriamycin before the tissue sample was taken. Calu-3 has a hypotriploid stemline chromosome number and expresses antigens blood type A and Rh+. Calu-3 cells display an epithelial morphology and adherent cultural properties. The Calu-3 cell line can be used to study lung cancer by following in vivo or in vitro transfection methods, and Altogen Biosystems has a Calu-3 xenograft model and a Calu-3 Transfection Reagent commercially available.